GTF2E1 Antibody - C-terminal region : FITC (P100892_P050-FITC)

Product Specifications

Gene Name

General transcription factor IIE, polypeptide 1, alpha 56kDa

Gene Aliases

FE, TF2E1, TFIIE-A

Gene ID

2960

Swiss Prot

P29083

Accession Number

NP_005504

Host

Rabbit

Reactivity

Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Immunogen

The immunogen is a synthetic peptide directed towards the C terminal region of human GTF2E1

Target

The general transcription factor TFIIE has an essential role in eukaryotic transcription initiation together with RNA polymerase II and other general factors. Human TFIIE consists of two subunits of relative molecular mass 57,000 (TFIIE-alpha) and 34,000 (TFIIE-beta) and joins the preinitiation complex after RNA polymerase II and TFIIF. TFIIE-alpha is necessary for transcription initiation together with TFIIE-beta, and recombinant TFIIE-alpha can fully replace the natural subunit in an in vitro transcription assay. Its sequence contains several interesting structural motifs (leucine repeat, zinc finger and helix-turn-helix) and sequence similarities to bacterial sigma factors that suggest direct involvement in the regulation of transcription initiation

Partner Proteins

UBC; AARSD1; ACBD3; XPO5; SH3GLB2; OXR1; NSUN2; RPUSD2; SNF8; EFTUD2; TTC1; SH3GL1; SARS; HARS; SRPK2; TCEA1; GTF2H1; GTF2E2; CDK7; APP; tat; GTF2F1; ELAVL1; ZSCAN1; ZBTB9; TRIM15; PCBD2; TRIM29; CBFA2T2; IKBKG; ATF7IP; TAF6; GTF2B; TRIM24; SND1; GTF2H4

Clonality

Polyclonal

Conjugation

FITC: Fluorescein Isothiocyanate

Type

Polyclonal Antibody

Applications

IHC, WB

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%

Format

Liquid. Purified antibody supplied in 1x PBS buffer.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.