GLS2 Antibody : FITC (OAPB01260-FITC)

Product Specifications

Gene Aliases

GA, GLS, LGA, hLGA

Gene ID

27165

Swiss Prot

Q9UI32

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

The immunogen for anti-GLS2 antibody: antibody was raised against a 18 amino acid peptide near the center terminus of human GLS2.

Target

Glutaminase 2

Clonality

Polyclonal

Conjugation

FITC: Fluorescein Isothiocyanate

Type

Polyclonal Antibody

Applications

ELISA, WB, IHC, IF

Purification

Affinity chromatography purified via peptide column

Concentration

1 mg/ml

Homology

Human; Mouse; Rat

Format

Liquid. Purified antibody supplied in 1x PBS buffer.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

References & Citations

Chung-Bok MI, Vincent N, Jhala U, et al. Rat hepatic glutaminase: identification of the full coding sequence and characterization of a functional promoter. Biochem. J. 1997; 324:193-200.; Gomez-Fabre PM, Aledo JC, del Castillo-Olivares A, et al. Molecular cloning, sequencing and expression studies of the human breast cancer cell glutaminase. Biochem. J. 2000; 345:365-75. ; Hu W, Zhang C, Wu R, et al. Glutaminase 2, a novel p53 target gene regulating energy metabolism and antioxidant function. Proc. Natl. Acad. Sci. USA 2010; 107:7455-60.

Shipping Conditions

Wet Ice

Specificity

GLS2 antibody is predicted to not cross-react with other catenin family members. Multiple isoforms of GLS2 are known to exist.

Applications Notes

GLS2 antibody can be used for detection of GLS2 by Western blot at 0.5 - 1 μ g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL.