C-raf Antibody : HRP (OAPB01117-HRP)
Product Specifications
Gene Aliases
NS5, CRAF, Raf-1, c-Raf, CMD1NN
Gene ID
5894
Swiss Prot
P04049
Host
Chicken
Reactivity
Human, Mouse, Rat
Immunogen
The immunogen for anti-C-raf antibody: antibody was raised against an 19 amino acid peptide near the amino terminus of human C-raf.
Target
V-raf murine sarcoma viral oncogene homolog 1
Clonality
Polyclonal
Conjugation
HRP: Horseradish Peroxidase
Type
Polyclonal Antibody
Applications
ELISA, WB
Purification
Affinity chromatography purified via peptide column
Concentration
0.6-0.7 mg/ml
Homology
Human; Mouse; Rat
Format
Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
Reconstitution
All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
References & Citations
Howe LR, Leevers SJ, Gomez N, et al. Activation of the MAP kinase pathway by the protein kinase raf. Cell1992; 71:335-42.; Chang F, Steelman LS, Shelton JG, et al. Regulation of cell cycle progression and apoptosis by the Ras/Raf/MEK/ERK pathway. Int. J. Oncol.2003; 22:469-80.; Pandit B, Sarkozy A, Pennacchio LA, et al. Gain-of-function RAF1 mutations cause Noonan and LEOPARD syndromes with hypertrophic cardiomyopathy. Nat. Genet.2007; 39:1007-12.
Shipping Conditions
Wet Ice
Applications Notes
C-raf antibody can be used for detection of C-raf by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
NCBI Gene Symbol
RAF1
NCBI GB Accession Number
RAF1
Host or Source
Chicken
Protein Name
RAF proto-oncogene serine/threonine-protein kinase
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