CDC42 Antibody : HRP (OAPB00856-HRP)
Product Specifications
Gene Aliases
TKS, G25K, CDC42Hs
Gene ID
998
Swiss Prot
P60953
Host
Chicken
Reactivity
Human, Mouse, Rat
Immunogen
The immunogen for anti-CDC42 antibody: antibody was raised against a 17 amino acid peptide near the amino terminus of human CDC42.
Target
Cell division cycle 42
Clonality
Polyclonal
Conjugation
HRP: Horseradish Peroxidase
Type
Polyclonal Antibody
Applications
ELISA, WB
Purification
Affinity chromatography purified via peptide column
Concentration
0.6-0.7 mg/ml
Homology
Human; Mouse; Rat
Format
Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
Reconstitution
All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
References & Citations
Erickson JW and Cerione RA. Multiple roles for Cdc42 in cell regulation. Curr. Opin. Cell Biol.2001; 13:153-7.; Chen W, Lim HH, and Lim L. The CDC42 homologue from Caenorhabditis elegans. Complementation of yeast mutation. J. Biol. Chem.1993; 268:13280-5.; Hart MJ, Eva A, Evans T, et al. Catalysis of guanine nucleotide exchange on the CDC42Hs protein by the dbl oncogene product. Nature1991; 354:311-4.; Rohatgi R, Ma L, Miki H, et al. The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly. Cell1999; 97:221-31.
Shipping Conditions
Wet Ice
Applications Notes
CDC42 antibody can be used for detection of CDC42 by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
NCBI Gene Symbol
CDC42
NCBI GB Accession Number
CDC42
Host or Source
Chicken
Protein Name
Cell division control protein 42 homolog
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