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Aak1 Antibody : HRP (OAPB00768-HRP)

Product Specifications

Gene Aliases

Aak1 (NT), AP2-associated protein kinase 1

Gene ID

22848

Swiss Prot

Q2M2I8

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

The immunogen for anti-Aak1 antibody: antibody was raised against a 20 amino acid peptide near the amino terminus of the human Aak1.

Target

(NT) AP2-associated protein kinase 1

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

ELISA, WB, ICC

Purification

Affinity chromatography purified via peptide column

Concentration

0.6-0.7 mg/ml

Homology

Human; Mouse; Rat

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

References & Citations

Connor SD and Schmid SL. Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis. J. Cell Biol.2002; 156:921-9.; Smythe E and Ayscough KR. The Ark1/Prk1 family of protein kinases. Regulators of endocytosis and the actin skeleton. EMBO Rep.2003; 4:246-51.; Ricotta D, Connor SD, Schmid SL, et al. Phosphorylation of the AP2 m2 subunit by AAK1 mediates high affinity binding to membrane protein sorting signals. J. Cell Biol.2002; 156:791-5.; Connor SD and Henderson DM. A novel AAK1 splice variant functions at multiple steps of the endocytic pathway. Mol. Biol. Cell2007; 18:2698-706.

Shipping Conditions

Wet Ice

Applications Notes

Aak1 antibody can be used for detection of Aak1 by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)

NCBI Gene Symbol

AAK1

NCBI GB Accession Number

AAK1

Host or Source

Rabbit

Protein Name

AP2-associated protein kinase 1

Frequently Asked Questions

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