SIRT2 Antibody : HRP (OAPB00636-HRP)
Product Specifications
Gene Aliases
SIR2, SIR2L, SIR2L2
Gene ID
22933
Swiss Prot
Q8IXJ6
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
The immunogen for anti-SIRT2 antibody: antibody was raised against a 19 amino acid peptide near the carboxy terminus of the human SIRT2.
Target
(CT) Sirtuin 2
Clonality
Polyclonal
Conjugation
HRP: Horseradish Peroxidase
Type
Polyclonal Antibody
Applications
ELISA, WB, IHC
Purification
Affinity chromatography purified via peptide column
Concentration
0.6-0.7 mg/ml
Homology
Human; Mouse; Rat
Format
Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
Reconstitution
All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
References & Citations
Yamamoto H, Schoonjans K, and Auwerx J. Sirtuin functions in health and disease. Mol. Endocrinol.2007; 21:1745-55.; Frye RA. Characterization of five human cDNAs with homology to the yeast SIR2 gene: SIR2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity. Biochem. Biophys. Res. Commun.1999; 260:273-279.; North BJ, Marshall BL, Borra MT, et. al. The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin deacetylase. Mol. Cell2003; 11:437-44.; Inoue T, Hiratsuka M, Osaki M, et al. The molecular biology of mammalian SIRT proteins: SIRT2 in cell cycle regulation. Cell Cycle2007; 6:1011-8.
Shipping Conditions
Wet Ice
Applications Notes
SIRT2 antibody can be used for detection of SIRT2 by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
NCBI Gene Symbol
SIRT2
NCBI GB Accession Number
SIRT2
Host or Source
Rabbit
Protein Name
NAD-dependent protein deacetylase sirtuin-2
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