Nhe-1 Antibody : HRP (OAPB00594-HRP)
Product Specifications
Gene Aliases
APNH, NHE1, LIKNS, NHE-1, PPP1R143
Gene ID
6548
Swiss Prot
P19634
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
The immunogen for anti-Nhe-1 antibody: antibody was raised against a 20 amino acid peptide near the carboxy terminus of the human Nhe-1.
Target
(NT) Sodium-hydrogen exchanger 1
Clonality
Polyclonal
Conjugation
HRP: Horseradish Peroxidase
Type
Polyclonal Antibody
Applications
ELISA, WB, IHC
Purification
Affinity chromatography purified via peptide column
Concentration
0.6-0.7 mg/ml
Homology
Human; Mouse; Rat
Format
Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
Reconstitution
All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
References & Citations
Mendoza SA. The Na+-H+ antiport is a mediator of cell proliferation. Acta Paediatr. Scand.1987; 76:545-7.; Denker SP, Huang DC, Orlowski J, et al. Direct binding of the NA—H exchanger NHE1 to ERM proteins regulates the cortical cytoskeleton and cell shape independently of H (+) translocation. Mol. Cell.2000; 6:1425-36.; Cingolani HE, Rebolledo OR, Portiansky EL, et al. Regression of hypertensive myocardial fibrosis by NA (+) /H (+) exchange inhibition. Hypertension2003; 41:373-7.
Shipping Conditions
Wet Ice
Applications Notes
Nhe-1 antibody can be used for detection of Nhe-1 by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
NCBI Gene Symbol
SLC9A1
NCBI GB Accession Number
SLC9A1
Host or Source
Rabbit
Protein Name
Sodium/hydrogen exchanger 1
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