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TRIM25 Antibody : HRP (OAPB00567-HRP)

Product Specifications

Gene Aliases

TRIM25 (NT), Tripartite motif-containing 25, estrogen responsive finger protein, EFP

Swiss Prot

D3DTY9

Host

Rabbit

Reactivity

Human

Immunogen

The immunogen for anti-TRIM25 antibody: antibody was raised against a 15 amino acid peptide from near the amino terminus of human TRIM25.

Target

(NT) estrogen responsive finger protein

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

ELISA, WB, ICC

Purification

Affinity chromatography purified via peptide column

Concentration

0.6-0.7 mg/ml

Homology

Human

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

References & Citations

Inoue S, Orimo A, Hosoi T, et al. Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein. Proc. Natl. Acad. Sci. USA1993; 11117-20.; Orimo A, Inoue S, Minowa O, et al. Underdeveloped uterus and reduced estrogen responsiveness in mice with disruption of the estrogen-responsive finger protein gene, which is a direct target of estrogen receptor a. Proc. Natl. Acad. Sci. USA1999; 96:12027-32.; Thomson SD, Ali S, Pickles L, et al. Analysis of estrogen-responsive finger protein expression in benign and malignant human breast. Int. J. Cancer2001; 91:152-8.; Urano T, Saito T, Tsukui T, et al. Efp targets 14-3-3s for proteolysis and promotes breast tumour growth. Nature2002; 417:871-5.

Shipping Conditions

Wet Ice

Applications Notes

TRIM25 antibody can be used for detection of TRIM25 by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)

NCBI Gene Symbol

TRIM25

NCBI GB Accession Number

TRIM25

Host or Source

Rabbit

Protein Name

Tripartite motif-containing 25, isoform CRA_a

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