MICA Antibody : HRP (OAPB00542-HRP)
Product Specifications
Gene Aliases
MIC-A, PERB11.1
Gene ID
100507436
Swiss Prot
Q29983
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
The immunogen for anti-MICA antibody: antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human MICA.
Target
MHC class I chain-related protein A
Clonality
Polyclonal
Conjugation
HRP: Horseradish Peroxidase
Type
Polyclonal Antibody
Applications
ELISA, WB, IHC
Purification
Affinity chromatography purified via peptide column
Concentration
0.6-0.7 mg/ml
Homology
Human; Mouse
Format
Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
Reconstitution
All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
References & Citations
Rudolph MG, Stanfield RL and Wilson IA. How TCRs bind MHCs, peptides, and coreceptors. Annu. Rev. Immunol.2006; 24:419-66.; Bahram S, Bresnahan M, Geraghty DE, et al. A second lineage of mammalian major histocompatibility complex I genes. Proc. Natl. Acad. Sci. USA1994; 91:6259-63.; Bauer S, Groh V, Wu J, et al. Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science1999; 285:727-9.; Maccalli C, Pende D, Castelli C, et al. NKG2D engagement of colorectal cancer-specific T cells strengthens TCR-mediated antigen stimulation and elicits TCR independent anti-tumor activity. Eur. J. Immunol.2003; 33:2033-43.
Shipping Conditions
Wet Ice
Applications Notes
MICA antibody can be used for detection of MICA by Western blot at 0.5 - 1 ug/ml. (Optimal dilution should be determined by user)
NCBI Gene Symbol
MICA
NCBI GB Accession Number
MICA
Host or Source
Rabbit
Protein Name
MHC class I polypeptide-related sequence A
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