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HUWE1 Antibody : HRP (OAPB00527-HRP)

Product Specifications

Gene Name

E3 ubiquitin-protein ligase HUWE1

Gene Aliases

MULE, Ib772, LASU1, MRXST, UREB1, HECTH9, URE-B1, ARF-BP1, HSPC272

Gene ID

10075

Swiss Prot

Q7Z6Z7

Accession Number

NP_113584.3XP_005262022.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

The immunogen for anti-ARF-BP1 antibody: antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human ARF-BP1.

Target

ARF binding protein

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

ELISA, WB, ICC

Purification

Affinity chromatography purified via peptide column

Concentration

0.6-0.7 mg/ml

Homology

Human; Mouse; Rat

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

References & Citations

Gallagher SJ, Kefford RF and Rizos H. The ARF tumour suppressor. Int. J. Biochem. Cell Biol.2006; 38:1637-41.; Pomerantz J, Schreiber-Agus N, Leigeois NJ, et al. The Ink4a tumor suppressor gene product, p19ARF interacts with MDM2 and neutralizes MDM2’s inhibition of p53. Cell1998; 92:725-34.; Chen D, Kon N, Li M, et al. ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor. Cell2005; 121:1071-83.; Zhong Q, Gao W, Du F, et al. Mule/ARF-BP1, a BH3-only E3 ubiquitin ligase, catalyzes the polyubiquitination of Mcl-1 and regulates apoptosis. Cell2005; 121:1085-95.

Shipping Conditions

Wet Ice

Applications Notes

ARF-BP1 antibody can be used for detection of ARF-BP1 by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)

NCBI Gene Symbol

HUWE1

NCBI GB Accession Number

HUWE1

Host or Source

Rabbit

Protein Name

E3 ubiquitin-protein ligase HUWE1

Nucleotide Accession Number

NM_031407.6XM_005261965.2

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