HUWE1 Antibody : Biotin (OAPB00527-Biotin)
Product Specifications
Gene Name
E3 ubiquitin-protein ligase HUWE1
Gene Aliases
MULE, Ib772, LASU1, MRXST, UREB1, HECTH9, URE-B1, ARF-BP1, HSPC272
Gene ID
10075
Swiss Prot
Q7Z6Z7
Accession Number
NP_113584.3XP_005262022.1
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
The immunogen for anti-ARF-BP1 antibody: antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human ARF-BP1.
Target
ARF binding protein
Clonality
Polyclonal
Conjugation
Biotin
Type
Polyclonal Antibody
Applications
ELISA, WB, ICC
Purification
Affinity chromatography purified via peptide column
Concentration
1 mg/ml
Homology
Human; Mouse; Rat
Format
Liquid. Purified antibody supplied in 1x PBS buffer.
Reconstitution
All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
References & Citations
Gallagher SJ, Kefford RF and Rizos H. The ARF tumour suppressor. Int. J. Biochem. Cell Biol.2006; 38:1637-41.; Pomerantz J, Schreiber-Agus N, Leigeois NJ, et al. The Ink4a tumor suppressor gene product, p19ARF interacts with MDM2 and neutralizes MDM2’s inhibition of p53. Cell1998; 92:725-34.; Chen D, Kon N, Li M, et al. ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor. Cell2005; 121:1071-83.; Zhong Q, Gao W, Du F, et al. Mule/ARF-BP1, a BH3-only E3 ubiquitin ligase, catalyzes the polyubiquitination of Mcl-1 and regulates apoptosis. Cell2005; 121:1085-95.
Shipping Conditions
Wet Ice
Applications Notes
ARF-BP1 antibody can be used for detection of ARF-BP1 by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
NCBI Gene Symbol
HUWE1
NCBI GB Accession Number
HUWE1
Host or Source
Rabbit
Protein Name
E3 ubiquitin-protein ligase HUWE1
Nucleotide Accession Number
NM_031407.6XM_005261965.2
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