Beta-actin Antibody : HRP (OAPB00400-HRP)
Product Specifications
Gene Name
Actin, beta
Gene Aliases
BRWS1, PS1TP5BP1
Gene ID
60
Swiss Prot
P60709
Accession Number
AAH02409
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
The immunogen for anti-Beta-actin antibody: antibody was raised against a 16 amino acid peptide from near the carboxy-terminus of human b-actin.
Target
(CT) a major cytoskeletal protein, C Terminus
Clonality
Polyclonal
Conjugation
HRP: Horseradish Peroxidase
Type
Polyclonal Antibody
Applications
ELISA, WB, ICC
Purification
Affinity chromatography purified via peptide column
Concentration
0.6-0.7 mg/ml
Homology
Human; Mouse; Rat
Format
Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
Reconstitution
All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
References & Citations
Lambrechts A, Van Troys, M and Ampe C. The actin cytoskeleton in normal and pathological cell motility. Int. J. Biochem. Cell Biol. 2004; 36:1890-909.; Gourlay CW and Ayscough KR. The actin cytoskeleton: a key regulator of apoptosis and ageing. Nat. Rev. 2005; 6:583-9.
Shipping Conditions
Wet Ice
Applications Notes
B-actin antibody can be used for the detection of b-actin by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
NCBI Gene Symbol
ACTB
NCBI GB Accession Number
ACTB
Host or Source
Rabbit
Protein Name
Actin, cytoplasmic 1
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