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XBP1 Antibody : HRP (OAPB00381-HRP)

Selected Variant

OAPB00381-100UG-HRP

Size: 0.1 mg

Product Specifications

Gene Name

X-box-binding protein 1

Gene Aliases

XBP2, TREB5, XBP-1, TREB-5

Gene ID

7494

Swiss Prot

P17861

Accession Number

NP_001073007.1NP_005071.2

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

The immunogen for anti-XBP-1 antibody: antibody was raised against a 18 amino acid peptide from near the amino terminus of human XBP-1.

Target

(NT) An ER signaling protein of the unfolded protein response pathway

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

ELISA, WB, IHC

Purification

Affinity chromatography purified via peptide column

Concentration

0.6-0.7 mg/ml

Homology

Human; Mouse

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

References & Citations

Yoshida H, Matsui T, Yamamoto T, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1p in response to ER stress to produce a highly active transcription factor. Cell 2001; 107:881-91.; Calfon M, Zeng H, Urano F, et al. IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA. Nature 2002; 415:92-6.; Haze K, Yoshida H, Yanagi H, et al. Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic stress. Mol. Cell. Biol. 1999; 10:3787-99.; Little E, Ramakrishnan M, Roy B, et al. The glucose-regulated proteins (GRP78 and GRP94) : functions, gene regulation, and applications. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18.

Shipping Conditions

Wet Ice

Applications Notes

XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)

NCBI Gene Symbol

XBP1

NCBI GB Accession Number

XBP1

Host or Source

Rabbit

Protein Name

X-box-binding protein 1

Nucleotide Accession Number

NM_001079539.1NM_005080.3

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