ERN1 Antibody : HRP (OAPB00366-HRP)

Product Specifications

Gene Name

Serine/threonine-protein kinase/endoribonuclease IRE1

Gene Aliases

IRE1, IRE1P, IRE1a, hIRE1p

Gene ID

2081

Swiss Prot

O75460

Accession Number

NP_001424.3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

The immunogen for anti-IRE1p antibody: antibody was raised against a 15 amino acid peptide from near the amino terminus of human IRE1p.

Target

(NT) Endoplasmic reticulum-to-nucleus signaling 1

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

ELISA, WB, ICC

Purification

Affinity chromatography purified via peptide column

Concentration

0.6-0.7 mg/ml

Homology

Human; Mouse; Rat

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

References & Citations

Little E, Ramakrishnan M, Roy B, et al. The glucose-regulated proteins (GRP78 and GRP94) : functions, gene regulation, and applications. Crit. Rev. Eukaryot. Gene Expr.1994; 4:1-18. ; Lee AS. The ER chaperone and signaling regulator GRP78/BiP as a monitor of endoplasmic reticulum stress. Methods2005; 35:373-81.; Bertolotti A, Zhang Y, Hendershot LM, et al. Dynamic interaction of BiP and ER stress transducers in the unfolded-protein response. Nat. Cell Biol.2000; 2:326-32.; Shen J, Chen X, Hendershot L, et al. ER stress regulation of ATF6 localization by dissociation of BiP/GRP78 binding and unmasking of Golgi localization signals. Dev. Cell2002; 3:99-111.