TRIM5 Antibody : Biotin (OAPB00225-Biotin)

The immunogen for anti-TRIM5 alpha antibody: antibody was raised against a synthetic peptide corresponding to amino acids near the mid-region of rhesus monkey TRIM5 alpha.

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Reymond A, Meroni G, Fantozzi A, et al. The tripartite motif family identifies cell compartments. EMBO J. 2001; 20:2140-51.; Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53.; Hatziioannou T, Perez-Caballero D, Yang A, et al. Retrovirus resistance factors REF1 and Lv1 are species-specific variants of TRIM5alpha. Proc. Nat’l. Acad. Sci. USA 2004; 101:10774-9

TRIM5 alpha antibody can be used for detection of TRIM5 alpha by Western blot at 1 to 2 ug/ml. A band at approximately 55 kDa can be detected. (Optimal dilution should be determined by user)