TRIM5 Antibody : HRP (OAPB00224-HRP)
Product Specifications
Gene Name
Tripartite motif-containing protein 5
Gene Aliases
RNF88, TRIM5alpha
Gene ID
85363
Swiss Prot
Q9C035-1
Accession Number
NP_149023.2NP_149083.2NP_149084.2XP_005253240.1XP_005253241.1
Host
Rabbit
Reactivity
Human
Immunogen
The immunogen for anti-TRIM5 alpha antibody: antibody was raised against a synthetic peptide corresponding to amino acids near the C-terminus of rhesus monkey TRIM5 alpha.
Target
(CT) Retroviral restriction factor
Clonality
Polyclonal
Conjugation
HRP: Horseradish Peroxidase
Type
Polyclonal Antibody
Applications
ELISA, WB, IF
Purification
Affinity chromatography purified via peptide column
Concentration
0.6-0.7 mg/ml
Homology
Human
Format
Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
Reconstitution
All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
References & Citations
Reymond A, Meroni G, Fantozzi A, et al. The tripartite motif family identifies cell compartments. EMBO J. 2001; 20:2140-51.; Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53.; Hatziioannou T, Perez-Caballero D, Yang A, et al. Retrovirus resistance factors REF1 and Lv1 are species-specific variants of TRIM5alpha. Proc. Nat’l. Acad. Sci. USA 2004; 101:10774-9
Shipping Conditions
Wet Ice
Specificity
Anti-TRIM5 alpha is human specific but should presumably also react with rhesus protein.
Applications Notes
TRIM5 alpha antibody can be used for detection of TRIM5 alpha by Western blot at 1 to 2 ug/ml. A band at approximately 55 kDa can be detected. Anti-TRIM5 alpha is human specific but should presumably also react with rhesus protein. (Optimal dilution should be determined by user)
NCBI Gene Symbol
TRIM5
NCBI GB Accession Number
TRIM5
Host or Source
Rabbit
Protein Name
Tripartite motif-containing protein 5
Nucleotide Accession Number
NM_033034.2NM_033092.2NM_033093.2XM_005253183.1XM_005253184.1
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