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NLRC4 Antibody : HRP (OAPB00186-HRP)

Selected Variant

OAPB00186-100UG-HRP

Size: 0.1 mg

Product Specifications

Gene Name

NLR family CARD domain-containing protein 4

Gene Aliases

CLAN, IPAF, AIFEC, CLAN1, CLANA, CLANB, CLANC, CLAND, FCAS4, CARD12, CLR2.1

Gene ID

58484

Swiss Prot

Q9NPP4

Accession Number

NP_001186067.1NP_001186068.1NP_001289433.1NP_067032.3

Host

Rabbit

Reactivity

Human

Immunogen

The immunogen for anti-Ipaf antibody: antibody was raised against a synthetic peptide corresponding to amino acids near the C-terminus of human Ipaf.

Target

ICE protease activating factor

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

ELISA, WB, ICC

Purification

Affinity chromatography purified via peptide column

Concentration

0.6-0.7 mg/ml

Homology

Human

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

References & Citations

Li P, Nijhawan D, Budihardjo I, et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997; 91:479-89.; Poyet J-L, Srinivaula SM, Tnani M, et al. Identification of Ipaf, a human caspase-1-activating protein related to Apaf-1. J. Biol. Chem. 2001; 276:28309-13.; Geddes BJ, Wang L, Huang WJ, et al. Human CARD12 is a novel CED4/Apaf-1 family member that induces apoptosis. Biochem. Biophys. Res. Commun. 2001; 284:77-82.

Shipping Conditions

Wet Ice

Applications Notes

Ipaf polyclonal antibody can be used for the detection of Ipaf by Western blot at 0.5 to 2 ug/ml. Human peripheral blood leukocyte (PBL) cell lysate can be used as positive control and a 110 kDa band can be detected. Ipaf antibody is human specific. (Optimal dilution should be determined by user)

NCBI Gene Symbol

NLRC4

NCBI GB Accession Number

NLRC4

Host or Source

Rabbit

Protein Name

NLR family CARD domain-containing protein 4

Nucleotide Accession Number

NM_001199138.1NM_001199139.1NM_001302504.1NM_021209.4

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