IKK gamma Antibody : FITC (OAPB00122-FITC)

The immunogen for anti-IKK gamma antibody: antibody was raised against a peptide corresponding to amino acids 400 to 416 of human IKK gamma, which are identical to those of mouse homologue.

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Rothwarf DM, Zandi E, Natoli G, Karin M. IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex. Nature 1998;395 (6699) :297-300; Yamaoka S, Courtois G, Bessia C, Whiteside ST, Weil R, Agou F, Kirk HE, Kay RJ, Israel A. Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation. Cell. 1998;93 (7) :1231-40. ; Li Y, Kang J, Friedman J, Tarassishin L, Ye J, Kovalenko A, Wallach D, Horwitz MS. Identification of a cell protein (FIP-3) as a modulator of NF-kappaB activity and as a target of an adenovirus inhibitor of tumor necrosis factor alpha-induced apoptosis. Proc Natl Acad Sci U S A 1999;96 (3) :1042-7 ; Mercurio F, Murray BW, Shevchenko A, Bennett BL, Young DB, Li JW, Pascual G, Motiwala A, Zhu H, Mann M, Manning AM. IkappaB kinase (IKK) -associated protein 1, a common component of the heterogeneous IKK complex. Mol Cell Biol. 1999;19 (2) :1526-38.

IKK gamma antibody can be used for detection of IKK gamma by Western blot at 0.5 to 1 ug/ml. A 52 kDa band should be detected. IKK gamma has no cross response to IKK alpha or IKK beta. (Optimal dilution should be determined by user)