STS Antibody : HRP (OAPB00111-HRP)

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Masumoto J, Taniguchi S, Ayukawa K, Sarvotham H, Kishino T, Niikawa N, Hidaka E, Katsuyama T, Higuchi T, Sagara J. ASC, a novel 22-kDa protein, aggregates during apoptosis of human promyelocytic leukemia HL-60 cells. J Biol Chem. 1999;274 (48) :33835-8. ; Masumoto J, Taniguchi S, Nakayama K, Ayukawa K, Sagara J. Murine Ortholog of ASC, a CARD-Containing Protein, Self-Associates and Exhibits Restricted Distribution in Developing Mouse Embryos. Exp Cell Res. 2001;262 (2) :128-133.; Conway KE, McConnell BB, Bowring CE, Donald CD, Warren ST , Vertino PM. TMS1, a novel proapoptotic caspase recruitment domain protein, is a target of methylation-induced gene silencing in human breast cancers. Cancer Res. 2000;60 (22) :6236-42. ; McConnell BB, Vertino PM. Activation of a caspase-9-mediated apoptotic pathway by subcellular redistribution of the novel caspase recruitment domain protein TMS1. Cancer Res. 2000;60 (22) :6243-7. (WD0102)

ASC antibody can be used for detection of ASC/TMS1 by Western blot at 1 ug/ml. An approximately 25 kDa band can be detected. (Optimal dilution should be determined by user)