CASP8AP2 Antibody : HRP (OAPB00106-HRP)
Product Specifications
Gene Name
CASP8-associated protein 2
Gene Aliases
CED-4, FLASH, RIP25
Gene ID
9994
Swiss Prot
Q9UKL3
Accession Number
NP_001131139.1NP_001131140.1NP_036247.1
Host
Rabbit
Reactivity
Human
Immunogen
The immunogen for anti-FLASH antibody: antibody was raised against a synthetic peptide corresponding to amino acids near the carboxy-terminus of human FLASH which differ from those of mouse by one amino acid.
Target
(CT) Homolog of CED4
Clonality
Polyclonal
Conjugation
HRP: Horseradish Peroxidase
Type
Polyclonal Antibody
Applications
ELISA, WB, ICC
Purification
Affinity chromatography purified via peptide column
Concentration
0.6-0.7 mg/ml
Homology
Human
Format
Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
Reconstitution
All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
References & Citations
Imai Y, Kimura T, Murakami A, Yajima N, Sakamaki K, Yonehara S. The CED-4-homologous protein FLASH is involved in Fas-mediated activation of caspase-8 during apoptosis. Nature 1999;398:777-85 ; Koonin EV, Aravind L, Hofmann K, Tschopp J, Dixit VM Apoptosis. Searching for FLASH domains. Nature 1999;401:662-3 ; Medema JP. Apoptosis. Life and death in a FLASH. Nature 1999;398:756-7 (WD0800)
Shipping Conditions
Wet Ice
Applications Notes
FLASH antibody can be used for detection of FLASH by Western blot at 0.25 to 0.5 ug/ml. An approximate 220 kDa band can be detected. (Optimal dilution should be determined by user)
NCBI Gene Symbol
CASP8AP2
NCBI GB Accession Number
CASP8AP2
Host or Source
Rabbit
Protein Name
CASP8-associated protein 2
Nucleotide Accession Number
NM_001137667.1NM_001137668.1NM_012115.3
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