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DFFB Antibody : FITC (OAPB00075-FITC)

Product Specifications

Gene Name

DNA fragmentation factor subunit beta

Gene Aliases

CAD, CPAN, DFF2, DFF40, DFF-40

Gene ID

1677

Swiss Prot

O76075

Accession Number

NP_004393.1

Host

Rabbit

Reactivity

Human

Immunogen

The immunogen for anti-DFF40 antibody: antibody was raised against a peptide corresponding to amino acids 3 to 18 of human DFF40.

Target

(NT) Caspase Activated DNase

Clonality

Polyclonal

Conjugation

FITC: Fluorescein Isothiocyanate

Type

Polyclonal Antibody

Applications

ELISA, WB, ICC

Purification

Affinity chromatography purified via peptide column

Concentration

1 mg/ml

Homology

Human

Format

Liquid. Purified antibody supplied in 1x PBS buffer.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

References & Citations

Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50; Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99 ; Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L, Williams LT. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45. Curr Biol 1998;8:537-40; Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-6

Shipping Conditions

Wet Ice

Applications Notes

DFF40 antibody can be used for detection of DFF40 by Western blot at 1:500 to 1:2000 dilution. A 40 kDa band can be detected. (Optimal dilution should be determined by user)

NCBI Gene Symbol

DFFB

NCBI GB Accession Number

DFFB

Host or Source

Rabbit

Protein Name

DNA fragmentation factor subunit beta

Nucleotide Accession Number

NM_004402.3

Frequently Asked Questions

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