DFFB Antibody : FITC (OAPB00075-FITC)
Product Specifications
Gene Name
DNA fragmentation factor subunit beta
Gene Aliases
CAD, CPAN, DFF2, DFF40, DFF-40
Gene ID
1677
Swiss Prot
O76075
Accession Number
NP_004393.1
Host
Rabbit
Reactivity
Human
Immunogen
The immunogen for anti-DFF40 antibody: antibody was raised against a peptide corresponding to amino acids 3 to 18 of human DFF40.
Target
(NT) Caspase Activated DNase
Clonality
Polyclonal
Conjugation
FITC: Fluorescein Isothiocyanate
Type
Polyclonal Antibody
Applications
ELISA, WB, ICC
Purification
Affinity chromatography purified via peptide column
Concentration
1 mg/ml
Homology
Human
Format
Liquid. Purified antibody supplied in 1x PBS buffer.
Reconstitution
All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
References & Citations
Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50; Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99 ; Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L, Williams LT. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45. Curr Biol 1998;8:537-40; Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-6
Shipping Conditions
Wet Ice
Applications Notes
DFF40 antibody can be used for detection of DFF40 by Western blot at 1:500 to 1:2000 dilution. A 40 kDa band can be detected. (Optimal dilution should be determined by user)
NCBI Gene Symbol
DFFB
NCBI GB Accession Number
DFFB
Host or Source
Rabbit
Protein Name
DNA fragmentation factor subunit beta
Nucleotide Accession Number
NM_004402.3
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