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Apaf1 Antibody: HRP (OAPB00040-HRP)

Product Specifications

Gene Aliases

CED4, APAF-1

Gene ID

317

Swiss Prot

O14727

Accession Number

AAC51678

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

The immunogen for anti-Apaf1 antibody: antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human Apaf1. The sequence of the immunogenic peptide differs from that of murine Apaf1 by one amino acid.

Target

(CT) Apoptosis protease activating factor

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

ELISA, WB, IHC

Purification

Affinity chromatography purified via peptide column

Concentration

0.6-0.7 mg/mL

Homology

Human; Mouse; Rat

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Shipping Conditions

Wet Ice

Applications Notes

Apaf1 antibody can be used for detection of Apaf1 by Western blot at 0.5 - 1 ug/ml. Human heart tissue lysate can be used as positive control and a 130 kDa band should be detected. Apaf1 antibody is human, mouse and rat reactive. (Optimal dilution should be determined by user)

NCBI Gene Symbol

APAF1

Protein Name

Apoptotic protease-activating factor 1

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