TACE Antibody : Biotin (OAPB00018-Biotin)

The immunogen for anti-TACE antibody: antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human TACE. This sequence differs from those of mouse and rat TACE by one amino acid.

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Black RA, Rauch CT , Kozlosky CJ, et al. A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells. Nature 1997;385:729-733 ; Moss ML, Jin SL, Milla ME, et al. Cloning of a disintegrin metalloproteinase that processes precursor tumour-necrosis factor-alpha. Nature 1997;385:733-736; Mizui Y, Yamazaki K, Sagane K, Tanaka I. cDNA cloning of mouse tumor necrosis factor-alpha converting enzyme (TACE) and partial analysis of its promoter. Gene 1999;233:67-74 ; Peschon JJ, Slack JL, Reddy P, et al. An essential role for ectodomain shedding in mammalian development. Science 1998;282:1281-4

TACE antibody can be used for detection of TACE by Western blot at 1:500 to 1:2000 dilution. 80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated TACE. (Optimal dilution should be determined by user)