Eotaxin Antibody : Biotin (OAPB00014-Biotin)

The immunogen for anti-Eotaxin antibody: antibody was raised in rabbits against a peptide corresponding to amino acids near the carboxy terminus of human eotaxin.

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Kitaura M, Nakajima T, Imai T, et al. Molecular cloning of human eotaxin, an eosinophil-selective CC chemokine, and identification of a specific eosinophil eotaxin receptor, CC chemokine receptor 3. J Biol Chem 1996;271:7725-30 ; Ponath PD, Qin S, Ringler DJ, et al. Cloning of the human eosinophil chemoattractant, eotaxin. Expression, receptor binding, and functional properties suggest a mechanism for the selective recruitment of eosinophils. J Clin Invest 1996;97:604-12 ; He J, Chen Y, Farzan M, et al. CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia. Nature. 1997;385 (6617) :645-9 ; Choe H, Farzan M, Sun Y, et al. The beta-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates. Cell. 1996;85:1135-48 (WD0500)

Eotaxin antibody can be used for detection of eotaxin by Western blot at 0.5 to 1 ug/ml. The recombinant protein can be used as positive control and monomer and homodimer are detected at approximate 9 and 18 kDa. (Optimal dilution should be determined by user)