DOK1 Antibody : HRP (OAPB00006-HRP)

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Carpino N, Wisniewski D, Strife A, Marshak D, Kobayashi R, Stillman B, Clarkson B p62 (dok) : a constitutively tyrosine-phosphorylated, GAP-associated protein in chronic myelogenous leukemia progenitor cells. Cell 1997;88:197-204. ; Yamanashi Y, Baltimore D Identification of the Abl- and rasGAP-associated 62 KDa protein as a docking protein, Dok. Cell 1997;88:205-211.; Holland SJ, Gale NW, Gish GD, Roth RA, Songyang Z, Cantley LC, Henkemeyer M, Yancopoulos GD, Pawson T. Juxtamembrane tyrosine residues couple the Eph family receptor EphB2/Nuk to specific SH2 domain proteins in neuronal cells. EMBO J 1997;16:3877-3888.; Di Cristofano A, Carpino N, Dunant N, Friedland G, Kobayashi R, Strife A, Wisniewski D, Clarkson B, Pandolfi PP, Resh MD. Molecular cloning and characterization of p56 (dok-2) defines a new family of RasGAP-binding proteins. J Biol Chem 1998;273:4827-4830.

STAT1 alpha antibody can be used for Western blot at 0.5 - 1 ug and for immunoprecipitation at 2 to 4 ug per sample. Antibody can also be used for immunocytochemistry starting at 10 ug/mL and Immunohistochemistry starting at 2.5 ug/mL.