EIF3A Antibody : HRP (OABF01336-HRP)

Product Specifications

Gene Name

Eukaryotic translation initiation factor 3, subunit A

Gene Aliases

EIF3, P167, p180, p185, TIF32, EIF3S10, eIF3-p170, eIF3-theta

Gene ID

8661

Swiss Prot

Q14152

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

KLH conjugated synthetic peptide derived from human eIF3A

Target

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC) . The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

IHC-P, IF

Purification

Purified by Protein A.

Concentration

0.6-0.7 mg/ml

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.