EIF4E Antibody (Phospho-Ser209) : HRP (OABF00979-HRP)

Product Specifications

Gene Name

Eukaryotic translation initiation factor 4E

Gene Aliases

CBP, EIF4F, AUTS19, EIF4E1, eIF-4E, EIF4EL1

Gene ID

1977

Swiss Prot

P06730

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

KLH conjugated synthetic phosphopeptide derived from human eIF4E around the phosphorylation site of Ser209 [SG (p-S) TT]

Target

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

WB, IHC-P, IF

Purification

Purified by Protein A.

Concentration

0.6-0.7 mg/ml

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.