HIST1H2AB Antibody (Phospho-Thr121) : HRP (OAAF00510-HRP)
Product Specifications
Gene Name
Histone H2A type 1-B/E
Gene Aliases
H2A.1, H2A.2, H2A/a, H2AC4, H2AFA, HIST1H2AE
Gene ID
3012
Swiss Prot
P0C0S8P04908P20671Q96KK5Q6FI13Q7L7L0
Accession Number
NP_254280.1
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
The antiserum was produced against synthesized peptide derived between 81-130 amino acids from the C-Terminal region of human Histone H2A around the phosphorylation site of Thr121.
Clonality
Polyclonal
Conjugation
HRP: Horseradish Peroxidase
Type
Polyclonal Antibody
Applications
IHC, ELISA
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Concentration
0.6-0.7 mg/mL
Dilution
The optimal working dilution should be determined by the end user.
Format
Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
Reconstitution
All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
Molecular Weight
14 kD
Shipping Conditions
Wet Ice
Specificity
Histone H2A (Phospho-Thr121) antibody detects endogenous levels of Histone H2A only when phosphorylated at threonine 121.
Applications Notes
IHC: 1:50~1:100; ELISA: 1:1000
NCBI Gene Symbol
HIST1H2AB
Protein Name
Histone H2A type 3
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