NOS2 Antibody (Phospho-Tyr151) : HRP (OAAF00500-HRP)
Product Specifications
Gene Name
Nitric oxide synthase, inducible
Gene Aliases
NOS, INOS, NOS2A, HEP-NOS
Gene ID
4843
Swiss Prot
P35228
Accession Number
NP_000616.3
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
The antiserum was produced against synthesized peptide derived between 117-166 amino acids from the N-Terminal region of human iNOS around the phosphorylation site of Tyr151.
Clonality
Polyclonal
Conjugation
HRP: Horseradish Peroxidase
Type
Polyclonal Antibody
Applications
IHC, ELISA
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Concentration
0.6-0.7 mg/mL
Dilution
The optimal working dilution should be determined by the end user.
Format
Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
Reconstitution
All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
Molecular Weight
131 kD
Shipping Conditions
Wet Ice
Specificity
INos (Phospho-Tyr151) antibody detects endogenous levels of Inos only when phosphorylated at tyrosine 151.
Applications Notes
IHC: 1:50~1:100; ELISA: 1:10000
NCBI Gene Symbol
NOS2
Protein Name
Nitric oxide synthase, inducible
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