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RAF1 Antibody (Phospho-Thr269) : HRP (OAAF00476-HRP)

Product Specifications

Gene Name

RAF proto-oncogene serine/threonine-protein kinase

Gene Aliases

NS5, CRAF, Raf-1, c-Raf, CMD1NN

Gene ID

5894

Swiss Prot

P04049

Accession Number

NP_002871.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived between 231-280 amino acids from the Internal region of human C-RAF around the phosphorylation site of Thr269.

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

IHC, ELISA

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

0.6-0.7 mg/mL

Dilution

The optimal working dilution should be determined by the end user.

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Shipping Conditions

Wet Ice

Specificity

C-RAF (Phospho-Thr269) antibody detects endogenous levels of C-RAF only when phosphorylated at threonine 269.

Applications Notes

IHC: 1:50~1:100; ELISA: 1:40000

NCBI Gene Symbol

RAF1

Protein Name

RAF proto-oncogene serine/threonine-protein kinase

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