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AKT1 Antibody (Bi-Phospho-Tyr437/434) : FITC (OAAF00431-FITC)

Product Specifications

Gene Name

RAC-alpha serine/threonine-protein kinase

Gene Aliases

AKT, PKB, RAC, PRKBA, PKB-ALPHA, RAC-ALPHA

Gene ID

207

Swiss Prot

P31749Q9Y243

Accession Number

NP_001193658.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived between 406-455 amino acids from the C-Terminal region of human AKT1/3 around the phosphorylation site of Tyr437/434.

Clonality

Polyclonal

Conjugation

FITC: Fluorescein Isothiocyanate

Type

Polyclonal Antibody

Applications

WB, IHC, ELISA

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/mL

Dilution

The optimal working dilution should be determined by the end user.

Format

Liquid. Purified antibody supplied in 1x PBS buffer.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Shipping Conditions

Wet Ice

Specificity

AKT1/3 (Phospho-Tyr437/434) antibody detects endogenous levels of AKT1/3 only when phosphorylated at tyrosine 437.

Applications Notes

WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:20000

NCBI Gene Symbol

AKT1

Protein Name

RAC-gamma serine/threonine-protein kinase

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