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PDPK1 Antibody (Phospho-Tyr9) : HRP (OAAF00420-HRP)

Product Specifications

Gene Name

3-phosphoinositide-dependent protein kinase 1

Gene Aliases

PDK1, PDPK2, PDPK2P, PRO0461

Gene ID

5170

Swiss Prot

O15530

Accession Number

NP_001248745.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived between 1-50 amino acids from the N-Terminal region of human PDK1 around the phosphorylation site of Tyr9.

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

IHC, ELISA

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

0.6-0.7 mg/mL

Dilution

The optimal working dilution should be determined by the end user.

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Shipping Conditions

Wet Ice

Specificity

PDK1 (Phospho-Tyr9) antibody detects endogenous levels of PDK1 only when phosphorylated at tyrosine 9.

Applications Notes

IHC: 1:50~1:100; ELISA: 1:40000

NCBI Gene Symbol

PDPK1

Protein Name

3-phosphoinositide-dependent protein kinase 1

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