KDR Antibody (Phospho-Tyr1214) : HRP (OAAF00394-HRP)
Selected Variant
OAAF00394-100UG-HRP
Size: 100 µg
Product Specifications
Gene Name
Vascular endothelial growth factor receptor 2
Gene Aliases
FLK1, CD309, VEGFR, VEGFR2
Gene ID
3791
Swiss Prot
P35968
Accession Number
NP_002244.1
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
The antiserum was produced against synthesized peptide derived between 1180-1229 amino acids from the C-Terminal region of human VEGFR2 around the phosphorylation site of Tyr1214.
Clonality
Polyclonal
Conjugation
HRP: Horseradish Peroxidase
Type
Polyclonal Antibody
Applications
WB, IHC, ELISA
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Concentration
0.6-0.7 mg/ml
Dilution
The optimal working dilution should be determined by the end user.
Format
Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
Reconstitution
All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
Molecular Weight
151 kD
Shipping Conditions
Wet Ice
Specificity
VEGFR2 (Phospho-Tyr1214) antibody detects endogenous levels of VEGFR2 only when phosphorylated at tyrosine 1214.
Applications Notes
WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:10000
NCBI Gene Symbol
KDR
NCBI GB Accession Number
KDR
Host or Source
Rabbit
Protein Name
Vascular endothelial growth factor receptor 2
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