PPP1R1B Antibody (Phospho-Thr34) : HRP (OAAF00304-HRP)
Selected Variant
OAAF00304-100UG-HRP
Size: 100 µg
Product Specifications
Gene Name
Protein phosphatase 1 regulatory subunit 1B
Gene Aliases
DARPP32, DARPP-32
Gene ID
84152
Swiss Prot
Q9UD71
Accession Number
NP_001229393.1
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
The antiserum was produced against synthesized peptide derived between 18-67 amino acids from the N-Terminal region of human DARPP-32 around the phosphorylation site of Thr34.
Clonality
Polyclonal
Conjugation
HRP: Horseradish Peroxidase
Type
Polyclonal Antibody
Applications
WB, IHC, IF, ELISA
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Concentration
0.6-0.7 mg/ml
Dilution
The optimal working dilution should be determined by the end user.
Format
Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
Reconstitution
All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
Molecular Weight
22 kD
Shipping Conditions
Wet Ice
Specificity
DARPP-32 (Phospho-Thr34) antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine 34.
Applications Notes
WB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:10000
NCBI Gene Symbol
PPP1R1B
NCBI GB Accession Number
PPP1R1B
Host or Source
Rabbit
Protein Name
Protein phosphatase 1 regulatory subunit 1B
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