PPP1R1B Antibody (Phospho-Thr34) : FITC (OAAF00304-FITC)
Product Specifications
Gene Name
Protein phosphatase 1 regulatory subunit 1B
Gene Aliases
DARPP32, DARPP-32
Gene ID
84152
Swiss Prot
Q9UD71
Accession Number
NP_001229393.1
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
The antiserum was produced against synthesized peptide derived between 18-67 amino acids from the N-Terminal region of human DARPP-32 around the phosphorylation site of Thr34.
Clonality
Polyclonal
Conjugation
FITC: Fluorescein Isothiocyanate
Type
Polyclonal Antibody
Applications
WB, IHC, IF, ELISA
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Concentration
1 mg/mL
Dilution
The optimal working dilution should be determined by the end user.
Format
Liquid. Purified antibody supplied in 1x PBS buffer.
Reconstitution
All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
Molecular Weight
22 kD
Shipping Conditions
Wet Ice
Specificity
DARPP-32 (Phospho-Thr34) antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine 34.
Applications Notes
WB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:10000
NCBI Gene Symbol
PPP1R1B
Protein Name
Protein phosphatase 1 regulatory subunit 1B
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