PRKCE Antibody (Phospho-Ser729) : HRP (OAAF00149-HRP)
Product Specifications
Gene Name
Protein kinase C epsilon type
Gene Aliases
PKCE, nPKC-epsilon
Gene ID
5581
Swiss Prot
Q02156
Accession Number
NP_005391.1
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
The antiserum was produced against synthesized peptide derived between 688-737 amino acids from the C-Terminal region of human PKC epsilon around the phosphorylation site of Ser729.
Clonality
Polyclonal
Conjugation
HRP: Horseradish Peroxidase
Type
Polyclonal Antibody
Applications
WB, ELISA
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
0.6-0.7 mg/mL
Dilution
The optimal working dilution should be determined by the end user.
Format
Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
Reconstitution
All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
Shipping Conditions
Wet Ice
Specificity
PKC ε (Phospho-Ser729) antibody detects endogenous levels of PKC ε only when phosphorylated at serine 729.
Applications Notes
WB: 1:500~1:3000; ELISA: 1:10000
NCBI Gene Symbol
PRKCE
Protein Name
Protein kinase C epsilon type
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