CHEK2 Antibody (Phospho-Thr383) : HRP (OAAF00144-HRP)
Product Specifications
Gene Name
Serine/threonine-protein kinase Chk2
Gene Aliases
CDS1, CHK2, LFS2, RAD53, hCds1, HuCds1, PP1425
Gene ID
11200
Swiss Prot
O96017
Accession Number
NP_001005735.1
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
The antiserum was produced against synthesized peptide derived between 356-405 amino acids from the Internal region of human Chk2 around the phosphorylation site of Thr383.
Clonality
Polyclonal
Conjugation
HRP: Horseradish Peroxidase
Type
Polyclonal Antibody
Applications
WB, IF, ELISA
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Concentration
0.6-0.7 mg/mL
Dilution
The optimal working dilution should be determined by the end user.
Format
Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
Reconstitution
All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
Shipping Conditions
Wet Ice
Specificity
Chk2 (Phospho-Thr383) antibody detects endogenous levels of Chk2 only when phosphorylated at threonine 383.
Applications Notes
WB: 1:500~1:1000; IF: 1:100~1:500; ELISA: 1:20000
NCBI Gene Symbol
CHEK2
Protein Name
Serine/threonine-protein kinase Chk2
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