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EIF4B Antibody (Phospho-Ser422) : HRP (OAAF00124-HRP)

Product Specifications

Gene Name

Eukaryotic translation initiation factor 4B

Gene Aliases

EIF-4B, PRO1843

Gene ID

1975

Swiss Prot

P23588

Accession Number

NP_001408.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived between 388-437 amino acids from the Internal region of human eIF4B around the phosphorylation site of Ser422.

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

WB, IHC, IF, ELISA

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

0.6-0.7 mg/mL

Dilution

The optimal working dilution should be determined by the end user.

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Shipping Conditions

Wet Ice

Specificity

EIF4B (Phospho-Ser422) antibody detects endogenous levels of eIF4B only when phosphorylated at serine 422.

Applications Notes

WB: 1:500~1:3000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:40000

NCBI Gene Symbol

EIF4B

Protein Name

Eukaryotic translation initiation factor 4B

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