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Nos1 Antibody (Phospho-Ser852) : HRP (OAAF00101-HRP)

Selected Variant

OAAF00101-100UG-HRP

Size: 100 µg

Product Specifications

Gene Name

Nitric oxide synthase, brain

Gene Aliases

BNOS

Gene ID

24598

Swiss Prot

P29476

Accession Number

NP_434686.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived between 818-867 amino acids from the Internal region of human n-NOS around the phosphorylation site of Ser852.

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

WB, IHC, IF, ELISA

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Concentration

0.6-0.7 mg/ml

Dilution

The optimal working dilution should be determined by the end user.

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Molecular Weight

160 kD

Shipping Conditions

Wet Ice

Specificity

N-NOS (Phospho-Ser852) antibody detects endogenous levels of n-NOS only when phosphorylated at serine 847.

NCBI Gene Symbol

NOS1

NCBI GB Accession Number

Nos1

Host or Source

Rabbit

Protein Name

Nitric oxide synthase, brain

Price

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