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POLR2A Antibody (Phospho-Ser1619) : HRP (OAAF00054-HRP)

Product Specifications

Gene Name

DNA-directed RNA polymerase II subunit RPB1

Gene Aliases

RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, NEDHIB, RpIILS, hsRPB1, hRPB220

Gene ID

5430

Swiss Prot

P24928

Accession Number

NP_000928.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived between 1585-1634 amino acids from the C-Terminal region of human POLR2A around the phosphorylation site of Ser1619.

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

WB, IHC, IF, ELISA

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

0.6-0.7 mg/mL

Dilution

The optimal working dilution should be determined by the end user.

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Shipping Conditions

Wet Ice

Specificity

POLR2A (phospho-Ser1619) antibody detects endogenous levels of POLR2A only when phosphorylated at serine 1619.

Applications Notes

WB: 1: 500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:10000

NCBI Gene Symbol

POLR2A

Protein Name

DNA-directed RNA polymerase II subunit RPB1

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