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POLR2A Antibody (Phospho-Ser1619) : FITC (OAAF00054-FITC)

Product Specifications

Gene Name

DNA-directed RNA polymerase II subunit RPB1

Gene Aliases

RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, NEDHIB, RpIILS, hsRPB1, hRPB220

Gene ID

5430

Swiss Prot

P24928

Accession Number

NP_000928.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived between 1585-1634 amino acids from the C-Terminal region of human POLR2A around the phosphorylation site of Ser1619.

Clonality

Polyclonal

Conjugation

FITC: Fluorescein Isothiocyanate

Type

Polyclonal Antibody

Applications

WB, IHC, IF, ELISA

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/mL

Dilution

The optimal working dilution should be determined by the end user.

Format

Liquid. Purified antibody supplied in 1x PBS buffer.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Shipping Conditions

Wet Ice

Specificity

POLR2A (phospho-Ser1619) antibody detects endogenous levels of POLR2A only when phosphorylated at serine 1619.

Applications Notes

WB: 1: 500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:10000

NCBI Gene Symbol

POLR2A

Protein Name

DNA-directed RNA polymerase II subunit RPB1

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