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PRKAA1 Antibody (Phospho-Thr172) : Biotin (OAAF00003-Biotin)

Product Specifications

Gene Name

5'-AMP-activated protein kinase catalytic subunit alpha-1

Gene Aliases

AMPK, AMPKa1, AMPK alpha 1

Gene ID

5562

Swiss Prot

Q13131

Accession Number

NP_006242.5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived between 140-189 amino acids from the Internal region of human AMPK alpha around the phosphorylation site of Thr172.

Clonality

Polyclonal

Conjugation

Biotin

Type

Polyclonal Antibody

Applications

WB, IHC, ELISA

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/mL

Dilution

The optimal working dilution should be determined by the end user.

Format

Liquid. Purified antibody supplied in 1x PBS buffer.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Shipping Conditions

Wet Ice

Specificity

AMPKα (Phospho-Thr172) antibody detects endogenous levels of AMPKα only when phosphorylated at threonine172 in both chain 1 and 2.

Applications Notes

WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:40000

NCBI Gene Symbol

PRKAA1

Protein Name

5'-AMP-activated protein kinase catalytic subunit alpha-1

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