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CDC25C Antibody - N-terminal region : HRP (AVARP03034_P050-HRP)

Selected Variant

AVARP03034_P050-HRP

Size: 100 µL

Product Specifications

Gene Name

Cell division cycle 25 homolog C (S. pombe)

Gene Aliases

CDC25, PPP1R60

Gene ID

995

Swiss Prot

P30307

Accession Number

NP_073720

Host

Rabbit

Reactivity

Human, Mouse, Rabbit

Immunogen

The immunogen is a synthetic peptide directed towards the N terminal region of human CDC25C

Target

CDC25C gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

Partner Proteins

CDC37; UBC; HSP90AA1; HSPA4; YWHAB; YWHAQ; YWHAZ; YWHAH; YWHAG; YWHAE; PKN1; SFN; MAPK9; PLK1; CHEK1; CHEK2; CDS1; MARK3; BRSK2; CDK2; PCNA; Cdk1; USP17L2; MAPK3; MAPK1; BRSK1; LZTS1; PLK3; NEDD4; PIN1; MAPK14; TP53; HRAS; LCK; CCNB1; CCNA2; SRC; SP1

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

WB

Sample Type

There is BioGPS gene expression data showing that CDC25C is expressed in Jurkat

Concentration

0.5 mg/ml

Homology

Human: 100%; Mouse: 91%; Rabbit: 83%

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Molecular Weight

45kDa

References & Citations

Bonnet, J., (2008) Biochem. Biophys. Res. Commun. 370 (3), 483-488

Shipping Conditions

Wet Ice

Protein Length

400

NCBI Gene Symbol

CDC25C

NCBI GB Accession Number

CDC25C

Host or Source

Rabbit

Protein Name

M-phase inducer phosphatase 3

Nucleotide Accession Number

NM_022809

Peptide Sequence

QKYEKLEKIGEGTYGTVFKAKNRETHEIVALKRVRLDDDDEGVPSSALRE

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