Nae1 Antibody - C-terminal region : FITC (ARP59379_P050-FITC)
Product Specifications
Gene Name
NEDD8 activating enzyme E1 subunit 1
Gene Aliases
Ap, 59kDa, Appbp1
Gene ID
234664
Swiss Prot
Q8VBW6
Accession Number
NP_659180
Host
Rabbit
Reactivity
Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Zebrafish
Immunogen
The immunogen is a synthetic peptide directed towards the C-terminal region of Mouse Nae1
Target
Nae1 is a regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.
Clonality
Polyclonal
Conjugation
FITC: Fluorescein Isothiocyanate
Type
Polyclonal Antibody
Applications
WB
Purification
Affinity Purified
Concentration
0.5 mg/ml
Homology
Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 79%
Format
Liquid. Purified antibody supplied in 1x PBS buffer.
Reconstitution
All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
Molecular Weight
60kDa
Shipping Conditions
Wet Ice
Product Datasheet
Anti-Nae1 (ARP59379_P050-FITC) antibody
Protein Length
534
NCBI Gene Symbol
NAE1
NCBI GB Accession Number
Nae1
Host or Source
Rabbit
Protein Name
NEDD8-activating enzyme E1 regulatory subunit
Nucleotide Accession Number
NM_144931
Peptide Sequence
ARALKEFVAKEGQGNLPVRGTIPDMIADSNKYIKLQNVYREKAKKDAAAV
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