Nae1 Antibody - C-terminal region : Biotin (ARP59379_P050-Biotin)
Selected Variant
ARP59379_P050-Biotin
Size: 100 µL
Product Specifications
Gene Name
NEDD8 activating enzyme E1 subunit 1
Gene Aliases
Ap, 59kDa, Appbp1
Gene ID
234664
Swiss Prot
Q8VBW6
Accession Number
NP_659180
Host
Rabbit
Reactivity
Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Zebrafish
Immunogen
The immunogen is a synthetic peptide directed towards the C-terminal region of Mouse Nae1
Target
Nae1 is a regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.
Clonality
Polyclonal
Conjugation
Biotin
Type
Polyclonal Antibody
Applications
WB
Purification
Affinity Purified
Concentration
0.5 mg/ml
Homology
Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 79%
Format
Liquid. Purified antibody supplied in 1x PBS buffer.
Reconstitution
All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
Molecular Weight
60kDa
Shipping Conditions
Wet Ice
Protein Length
534
NCBI Gene Symbol
NAE1
NCBI GB Accession Number
Nae1
Host or Source
Rabbit
Protein Name
NEDD8-activating enzyme E1 regulatory subunit
Nucleotide Accession Number
NM_144931
Peptide Sequence
ARALKEFVAKEGQGNLPVRGTIPDMIADSNKYIKLQNVYREKAKKDAAAV
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