MED14 Antibody - middle region : Biotin (ARP58139_P050-Biotin)

Product Specifications

Gene Name

Mediator complex subunit 14

Gene Aliases

CSRP, RGR1, CRSP2, EXLM1, CXorf4, CRSP150, DRIP150, TRAP170

Gene ID

9282

Swiss Prot

O60244

Accession Number

NP_004220

Host

Rabbit

Reactivity

Human, Mouse, Rabbit

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human MED14

Target

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation.

Partner Proteins

UBC; HECW2; CDK8; CDK19; MED12; ESR1; MED19; MED26; PPARG; FBXW7; EPAS1; MED11; MED8; MED29; MED18; MED4; PPP6R1; MED16; MED13; MED24; MED27; MED17; MED21; MED1; CTDP1; SREBF1; DCTN1; ACTN2; ACTN1; MED10; UACA; NECAB2; MED25; MED15; CRSP5; MED7; MED23; VD

Clonality

Polyclonal

Conjugation

Biotin

Type

Polyclonal Antibody

Applications

WB, CHIP

Purification

Affinity Purified

Sample Type

MED14 is strongly supported by BioGPS gene expression data to be expressed in A549

Concentration

0.5 mg/ml

Homology

Human: 100%; Mouse: 79%; Rabbit: 86%

Format

Liquid. Purified antibody supplied in 1x PBS buffer.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.