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APEX1 Antibody - middle region : FITC (ARP58028_P050-FITC)

Selected Variant

ARP58028_P050-FITC

Size: 100 µL

Product Specifications

Gene Name

APEX nuclease (multifunctional DNA repair enzyme) 1

Gene Aliases

APE, APX, APE1, APEN, APEX, HAP1, REF1

Gene ID

328

Swiss Prot

P27695

Accession Number

NP_001632

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human APEX1

Target

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.

Partner Proteins

GADD45A; UBC; RPA3; RPA2; RPA1; rev; CCDC124; RIC8A; PABPC1; TWF2; XPOT; HNRNPUL1; ARIH2; PAPSS2; NAE1; PSMG1; TXNRD1; TXN; TCEB1; LGALS1; HNRNPK; CAPNS1; SRPK2; SRPK1; MUTYH; HMGA1; HMGA2; GZMK; GZMA; YBX1; EP300; ASCL2; STAT3; SNRPD1; SFPQ; APP; UBE2I

Clonality

Polyclonal

Conjugation

FITC: Fluorescein Isothiocyanate

Type

Polyclonal Antibody

Applications

WB, IHC

Purification

Affinity Purified

Sample Type

APEX1 is supported by BioGPS gene expression data to be expressed in HEK293T

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Goat: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%

Format

Liquid. Purified antibody supplied in 1x PBS buffer.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Molecular Weight

35kDa

Shipping Conditions

Wet Ice

Protein Length

318

NCBI Gene Symbol

APEX1

NCBI GB Accession Number

APEX1

Host or Source

Rabbit

Protein Name

DNA- (apurinic or apyrimidinic site) lyase

Nucleotide Accession Number

NM_001641

Peptide Sequence

HEEIDLRNPKGNKKNAGFTPQERQGFGELLQAVPLADSFRHLYPNTPYAY

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