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EIF5 Antibody - N-terminal region : HRP (ARP53443_P050-HRP)

Product Specifications

Gene Name

Eukaryotic translation initiation factor 5

Gene Aliases

EIF-5, EIF-5A

Gene ID

1983

Swiss Prot

P55010

Accession Number

NP_892116

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the N terminal region of human EIF5

Target

EIF5 catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]) .

Partner Proteins

DUSP12; HUWE1; UBC; TUBB; UBFD1; SCLY; ISOC1; MAT2B; FERMT2; YWHAQ; STK24; YWHAZ; YWHAH; YWHAE; EIF4H; VCL; SNRPD1; PCYT2; PFAS; PDHB; PAFAH1B1; HSPA9; HNRNPK; GNAI3; GLA; G6PD; DFFA; TBCB; CAPNS1; ALDH7A1; LMNA; PUM1; NRGN; FN1; EIF3B; RPL29; APP; Nedd4

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

WB

Purification

Affinity Purified

Sample Type

There is BioGPS gene expression data showing that EIF5 is expressed in HeLa

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Molecular Weight

49kDa

References & Citations

Olsen, J.V., (2006) Cell 127 (3), 635-648

Shipping Conditions

Wet Ice

Protein Length

431

NCBI Gene Symbol

EIF5

NCBI GB Accession Number

EIF5

Host or Source

Rabbit

Protein Name

Eukaryotic translation initiation factor 5

Nucleotide Accession Number

NM_183004

Peptide Sequence

SVNVNRSVSDQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPT

Frequently Asked Questions

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