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MRPL10 Antibody - N-terminal region : HRP (ARP52830_P050-HRP)

Selected Variant

ARP52830_P050-HRP

Size: 100 µL

Product Specifications

Gene Name

Mitochondrial ribosomal protein L10

Gene Aliases

L10MT, MRPL8, RPML8, MRP-L8, MRP-L10

Gene ID

124995

Swiss Prot

Q7Z7H8

Accession Number

NP_660298

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog, Guinea Pig, Horse, Pig, Rabbit

Immunogen

The immunogen is a synthetic peptide directed towards the N terminal region of human MRPL10

Target

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 5q. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 5q.

Partner Proteins

FAM9B; KLHL12; PNMA1; TCF4; REL; UBC; NPM1; MRPL1; MRPL11; MRPL41; MRPL15; MRPL13; MRPL3; MRPL12; MRPL23; SLC25A3; ILF3; ICT1; HNRNPU; ABCC2; BCS1L; APP; ABCB7; MRPL10; USP22

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

WB

Purification

Affinity Purified

Concentration

0.5 mg/ml

Homology

Dog: 83%; Guinea Pig: 77%; Horse: 83%; Human: 100%; Mouse: 85%; Pig: 85%; Rabbit: 92%; Rat: 77%

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Molecular Weight

29kDa

References & Citations

Wang, C.C., (2004) Biochem. Biophys. Res. Commun. 314 (2), 335-350

Shipping Conditions

Wet Ice

Protein Length

261

NCBI Gene Symbol

MRPL10

NCBI GB Accession Number

MRPL10

Host or Source

Rabbit

Protein Name

39S ribosomal protein L10, mitochondrial

Nucleotide Accession Number

NM_145255

Peptide Sequence

HRRVMHFQRQKLMAVTEYIPPKPAIHPSCLPSPPSPPQEEIGLIRLLRRE

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