DNMT3B Antibody - middle region : FITC (ARP49124_P050-FITC)

Product Specifications

Gene Name

DNA (cytosine-5-) -methyltransferase 3 beta

Gene Aliases

ICF, ICF1, M.HsaIIIB

Gene ID

1789

Swiss Prot

Q9UBC3-3

Accession Number

NP_787045

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human DNMT3B

Target

DNMT3B is required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. DNMT3B may preferentially methylate nucleosomal DNA within the nucleosome core region. DNMT3B may function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. DNMT3B seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, DNMT3B is involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.

Partner Proteins

RBBP7; RBBP4; HDAC2; HDAC1; CHD4; MTA2; MTA1; POLR2H; POLR2C; POLR2A; DNMT1; LEO1; CDC73; PAF1; RTF1; CTR9; NRIP1; EZH2; DNMT3L; SUZ12; SIRT1; EED; UBC; EPHB1; CREB1; CBX5; TRIM28; ZFP57; UHRF2; UHRF1; MAP1LC3B; UBE2W; PLEKHJ1; DUSP23; CMTM6; PAM16; TSC22

Clonality

Polyclonal

Conjugation

FITC: Fluorescein Isothiocyanate

Type

Polyclonal Antibody

Applications

Purification

Affinity Purified

Sample Type

DNMT3B is supported by BioGPS gene expression data to be expressed in OVCAR3

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%

Format

Liquid. Purified antibody supplied in 1x PBS buffer.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.